The research unit specialised in the generation of trangenic animals has been active since May 2002. The service of generation of transgenic mice is available for all CEND research scientics. The technique of embryonic re-derivation is in preparation. We therefore have all the instrumentation necessary for microijection of cells. In addition, the CEND has reinforced this equipment with:
Two "Versadoc" for the aquisition and the quantitative analysis of data attained both from gels for nucleic acids and filters; thus it is no longer necessary to use a dark room. Phosphoimagers for the aquisition of data attained from filters prepared with radioactively labelled samples. OCD video camera for the analyses of luminescent and fluorescent samples. Moreover, a dark room is available where this CCD video camera can be used for the quantification of luminescence and flurescence in tissues and "in toto" animals.
Two real time PCR for the quantitative analysis of cDNA;
An instrument for the sequencing of DNA that can be used at the Departement of Neurological Sciences (responsible: Giacomo Comi).
One of the aims of this laboratoy is the diffusion of technological know-how and, therefore, it offers refresher courses about technologies used in this lab for students, young graduates and technicians. For longer stays in this lab, grants are available.